Auto‐inhibition of Mif2/CENP‐C ensures centromere‐dependent kinetochore assembly in budding yeast
نویسندگان
چکیده
منابع مشابه
Abnormal kinetochore structure activates the spindle assembly checkpoint in budding yeast.
Saccharomyces cerevisiae cells containing one or more abnormal kinetochores delay anaphase entry. The delay can be produced by using centromere DNA mutations present in single-copy or kinetochore protein mutations. This observation is strikingly similar to the preanaphase delay or arrest exhibited in animal cells that experience spontaneous or induced failures in bipolar attachment of one or mo...
متن کاملKinetochore structure and spindle assembly checkpoint signaling in the budding yeast, Saccharomyces cerevisiae.
The Spindle Assembly Checkpoint (SAC) delays the onset of anaphase until every chromosome is properly bioriented at the spindle equator. Mutations in SAC genes have been found in tumors and compromised SAC function can increase the incidence of some carcinomas in mice, providing further links between cancer etiology, chromosome segregation defects and aneuploidy. Here we review recent developme...
متن کاملDual mechanisms regulate the recruitment of spindle assembly checkpoint proteins to the budding yeast kinetochore
Recruitment of spindle assembly checkpoint (SAC) proteins by an unattached kinetochore leads to SAC activation. This recruitment is licensed by the Mps1 kinase, which phosphorylates the kinetochore protein Spc105 at one or more of its six MELT repeats. Spc105 then recruits the Bub3-Bub1 and Mad1-Mad2 complexes, which produce the inhibitory signal that arrests cell division. The strength of this...
متن کاملMolecular Analysis of Kinetochore-Microtubule Attachment in Budding Yeast
The complex series of movements that mediates chromosome segregation during mitosis is dependent on the attachment of microtubules to kinetochores, DNA-protein complexes that assemble on centromeric DNA. We describe the use of live-cell imaging and chromatin immunoprecipitation in S. cerevisiae to identify ten kinetochore subunits, among which are yeast homologs of microtubule binding proteins ...
متن کاملLive-cell analysis of kinetochore–microtubule interaction in budding yeast
Kinetochore capture and transport by spindle microtubules plays a crucial role in high-fidelity chromosome segregation, although its detailed mechanism has remained elusive. It has been difficult to observe individual kinetochore-microtubule interactions because multiple kinetochores are captured by microtubules during a short period within a small space. We have developed a method to visualize...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2020
ISSN: 0261-4189,1460-2075
DOI: 10.15252/embj.2019102938